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The two states are adopted by concerted movement of the nicotinysyl moieties of NAAD and NAD, Phe-170, and residues 224–228, which may be triggered by differential coordination of a magnesium ion to NAAD and NAD. coli NADS structures revealed unexpected dynamical rearrangements in the NAAD/NAD binding pocket upon NAAD-to-NAD conversion, which define a catalysis state and a substrate/product exchange state. Comparison with the Bacillus subtilis NADS showed pronounced chemical differences in the NAAD/NAD binding sites and less prominent differences in the ATP binding pockets. The structures disclosed two NAAD/NAD binding sites at the dimer interface and an adenosine triphosphate (ATP) binding site within each subunit. Here we describe the crystal structures of the ammonia-dependent homodimeric NADS from Escherichia coli alone and in complex with natural substrates and with the reaction product NAD. Nicotinamide adenine dinucleotide synthetases (NADS) catalyze the amidation of nicotinic acid adenine dinucleotide (NAAD) to yield the enzyme cofactor nicotinamide adenine dinucleotide (NAD). One such problem is multiple records of the same information.įortunately you can learn how to remove duplicates from an Excel 2010 sheet, which will help to prevent you from generating incorrect data. Whether it is coming from a previous version of a similar spreadsheet, or if you are consolidating information from another location, you might run into some problems with the data contained in the new document. Sometimes when you are creating a spreadsheet in Microsoft Excel 2010, that information is not created from scratch. While deleting individual rows to remove these duplicates is manageable when it only occurs a couple of times, it can be overwhelming when there are a high number of duplicates. 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